GAPDH
Gene Ontology Biological Process
- apoptotic process [IMP]
- carbohydrate metabolic process [IDA]
- cellular response to interferon-gamma [ISO]
- gluconeogenesis [IDA]
- glycolytic process [IDA]
- microtubule cytoskeleton organization [IDA]
- multicellular organismal development [ISO]
- negative regulation of translation [ISO]
- neuron apoptotic process [IDA, IMP]
- oxidation-reduction process [ISO]
- peptidyl-cysteine S-trans-nitrosylation [IDA]
- protein stabilization [IDA]
- response to ammonium ion [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- GAIT complex [ISO, ISS]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- lipid particle [ISO]
- membrane [ISO]
- microtubule cytoskeleton [IDA]
- mitochondrion [ISO]
- nuclear membrane [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
- ribonucleoprotein complex [ISO]
- vesicle [ISO]
RILPL1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
GOSPEL: a neuroprotective protein that binds to GAPDH upon S-nitrosylation.
We recently reported a cell death cascade whereby cellular stressors activate nitric oxide formation leading to S-nitrosylation of GAPDH that binds to Siah and translocates to the nucleus. The nuclear GAPDH/Siah complex augments p300/CBP-associated acetylation of nuclear proteins, including p53, which mediate cell death. We report a 52 kDa cytosolic protein, GOSPEL, which physiologically binds GAPDH, in competition with Siah, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RILPL1 GAPDH | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| GAPDH RILPL1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RILPL1 GAPDH | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - | |
| GAPDH RILPL1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID