BAIT
ORC2
RRR1, SIR5, origin recognition complex subunit 2, L000001776, YBR060C
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
GO Process (5)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
MCM7
CDC47, MCM DNA helicase complex subunit MCM7, L000002760, YBR202W
Component of the Mcm2-7 hexameric helicase complex; MCM2-7 primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
GO Process (8)
GO Function (10)
GO Component (7)
Gene Ontology Biological Process
- DNA replication initiation [IGI, IMP]
- DNA strand elongation involved in DNA replication [IGI, IMP]
- DNA unwinding involved in DNA replication [IDA]
- chromatin silencing at silent mating-type cassette [IMP]
- chromatin silencing at telomere [IMP]
- double-strand break repair via break-induced replication [IMP]
- nuclear DNA replication [IMP]
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication [IDA, IPI]
Gene Ontology Molecular Function- ATP binding [IDA]
- ATP-dependent 3'-5' DNA helicase activity [IDA]
- ATP-dependent DNA helicase activity [IDA]
- ATP-dependent four-way junction helicase activity [IDA]
- DNA helicase activity [IDA]
- DNA replication origin binding [IDA]
- chromatin binding [IDA]
- single-stranded DNA binding [IMP]
- single-stranded DNA-dependent ATP-dependent DNA helicase activity [IDA]
- single-stranded DNA-dependent ATPase activity [IDA]
- ATP binding [IDA]
- ATP-dependent 3'-5' DNA helicase activity [IDA]
- ATP-dependent DNA helicase activity [IDA]
- ATP-dependent four-way junction helicase activity [IDA]
- DNA helicase activity [IDA]
- DNA replication origin binding [IDA]
- chromatin binding [IDA]
- single-stranded DNA binding [IMP]
- single-stranded DNA-dependent ATP-dependent DNA helicase activity [IDA]
- single-stranded DNA-dependent ATPase activity [IDA]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1.
To initiate DNA replication, the origin recognition complex (ORC) and Cdc6 load an Mcm2-7 double hexamer onto DNA. Without ATP hydrolysis, ORC-Cdc6 recruits one Cdt1-bound Mcm2-7 hexamer, thus forming an ORC-Cdc6-Cdt1-Mcm2-7 (OCCM) helicase-loading intermediate. Here we report a 3.9-A structure of Saccharomyces cerevisiae OCCM on DNA. Flexible Mcm2-7 winged-helix domains (WHDs) engage ORC-Cdc6. A three-domain Cdt1 configuration embraces Mcm2, Mcm4, ... [more]
Nat. Struct. Mol. Biol. Feb. 13, 2017; 0(0); [Pubmed: 28191893]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
ORC2 MCM7 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 162579 |
Curated By
- BioGRID