BAIT

CYLD

BRSS, CDMT, CYLD1, CYLDI, EAC, MFT, MFT1, SBS, TEM, USPL2, HSPC057
cylindromatosis (turban tumor syndrome)
Homo sapiens
PREY

RARRES3

HRASLS4, HRSL4, PLA1/2-3, RIG1, TIG3
retinoic acid receptor responder (tazarotene induced) 3
GO Process (2)
GO Function (2)
GO Component (0)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Syndecan-4 negatively regulates antiviral signalling by mediating RIG-I deubiquitination via CYLD.

Lin W, Zhang J, Lin H, Li Z, Sun X, Xin D, Yang M, Sun L, Li L, Wang H, Chen D, Sun Q

Retinoic acid-inducible gene I (RIG-I) plays important roles in pathogen recognition and antiviral signalling transduction. Here we show that syndecan-4 (SDC4) is a RIG-I-interacting partner identified in a yeast two-hybrid screen. We find that SDC4 negatively regulates the RIG-I-mediated antiviral signalling in a feedback-loop control manner. The genetic evidence obtained by using knockout mice further emphasizes this biological role of ... [more]

Nat Commun Jun. 09, 2016; 7(0);11848 [Pubmed: 27279133]

Throughput

  • Low Throughput

Curated By

  • BioGRID