BAIT

USP21

USP16, USP23, RP11-297K8.3
ubiquitin specific peptidase 21
UPS Project
Homo sapiens
PREY

SYNE1

8B, ARCA1, C6orf98, CPG2, EDMD4, MYNE1, Nesp1, SCAR8, dJ45H2.2, RP1-130E4.2
spectrin repeat containing, nuclear envelope 1
Glioblastoma Project
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The centrosomal deubiquitylase USP21 regulates Gli1 transcriptional activity and stability.

Heride C, Rigden DJ, Bertsoulaki E, Cucchi D, De Smaele E, Clague MJ, Urbe S

USP21 is a centrosome-associated deubiquitylase (DUB) that has been implicated in the formation of primary cilia - crucial organelles for the regulation of the Hedgehog (Hh) signaling pathway in vertebrates. Here, we identify KCTD6 - a cullin-3 E3-ligase substrate adapter that has been previously linked to Hh signaling - as well as Gli1, the key transcription factor responsible for Hh ... [more]

J. Cell. Sci. Nov. 01, 2016; 129(21);4001-4013 [Pubmed: 27621083]

Throughput

  • Low Throughput

Curated By

  • BioGRID