Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Wuho Is a New Member in Maintaining Genome Stability through its Interaction with Flap Endonuclease 1.

Cheng IC, Chen BC, Shuai HH, Chien FC, Chen P, Hsieh TS

Replication forks are vulnerable to wayward nuclease activities. We report here our discovery of a new member in guarding genome stability at replication forks. We previously isolated a Drosophila mutation, wuho (wh, no progeny), characterized by a severe fertility defect and affecting expression of a protein (WH) in a family of conserved proteins with multiple WD40 repeats. Knockdown of WH ... [more]

PLoS Biol. Jan. 01, 2016; 14(1);e1002349 [Pubmed: 26751069]

Throughput

  • Low Throughput

Curated By

  • BioGRID