PREY

DNM2

CMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII, LCCS5
dynamin 2
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Mib1 modulates dynamin 2 recruitment via Snx18 to promote Dll1 endocytosis for efficient Notch signaling.

Okano M, Matsuo H, Nishimura Y, Hozumi K, Yoshioka S, Tonoki A, Itoh M

Notch signaling regulates normal development and tissue homeostasis. Ligand endocytosis plays critical roles in Notch signaling activation. Endocytic proteins such as epsin and dynamin participate in Notch ligand activity by mediating Notch ligand endocytosis. The ubiquitin ligase Mib1 also plays essential roles in Notch signaling via Notch ligand ubiquitination. However, the molecular links between Mib1 and endocytic proteins have not ... [more]

Genes Cells May. 01, 2016; 21(5);425-41 [Pubmed: 26923255]

Throughput

  • Low Throughput

Curated By

  • BioGRID