LPIN1
Gene Ontology Biological Process
- cellular lipid metabolic process [TAS]
- dephosphorylation [IBA]
- fatty acid catabolic process [IBA, ISS]
- glycerophospholipid biosynthetic process [TAS]
- phosphatidylcholine biosynthetic process [TAS]
- phosphatidylethanolamine biosynthetic process [TAS]
- phospholipid metabolic process [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IBA]
- small molecule metabolic process [TAS]
- triglyceride biosynthetic process [IBA, TAS]
- triglyceride mobilization [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CSNK1E
Gene Ontology Biological Process
- DNA repair [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- Wnt signaling pathway [IBA]
- circadian regulation of gene expression [ISS]
- endocytosis [IBA]
- mitotic cell cycle [TAS]
- peptidyl-serine phosphorylation [IBA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISS]
- protein phosphorylation [IDA, ISS]
- regulation of cell shape [IBA]
- regulation of circadian rhythm [ISS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The SCFβ-TRCP E3 ubiquitin ligase complex targets Lipin1 for ubiquitination and degradation to promote hepatic lipogenesis.
The SCF(β-TRCP) E3 ubiquitin ligase complex plays pivotal roles in normal cellular physiology and in pathophysiological conditions. Identification of β-transducin repeat-containing protein (β-TRCP) substrates is therefore critical to understand SCF(β-TRCP) biology and function. We used a β-TRCP-phosphodegron motif-specific antibody in a β-TRCP substrate screen coupled with tandem mass spectrometry and identified multiple β-TRCP substrates. One of these substrates was Lipin1, ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID