BAIT

MPEG1

MPS1, Mpg-1
macrophage expressed gene 1
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2.

McCormack RM, Lyapichev K, Olsson ML, Podack ER, Munson GP

Perforin-2 (MPEG1) is an effector of the innate immune system that limits the proliferation and spread of medically relevant Gram-negative, -positive, and acid fast bacteria. We show here that a cullin-RING E3 ubiquitin ligase (CRL) complex containing cullin-1 and βTrCP monoubiquitylates Perforin-2 in response to pathogen associated molecular patterns such as LPS. Ubiquitylation triggers a rapid redistribution of Perforin-2 and ... [more]

Elife Sep. 29, 2015; 4(0); [Pubmed: 26418746]

Throughput

  • Low Throughput

Curated By

  • BioGRID