Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions.

Shen JP, Zhao D, Sasik R, Luebeck J, Birmingham A, Bojorquez-Gomez A, Licon K, Klepper K, Pekin D, Beckett AN, Sanchez KS, Thomas A, Kuo CC, Du D, Roguev A, Lewis NE, Chang AN, Kreisberg JF, Krogan N, Qi L, Ideker T, Mali P

We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual guide RNAs in three cell lines, comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified, and these patterns replicated with combinatorial drugs at 75% precision. From these ... [more]

Nat. Methods Mar. 20, 2017; 0(); [Pubmed: 28319113]

Throughput

High Throughput

Ontology Terms

phenotype: growth abnormality (HP:0001507)
phenotype: viability (PATO:0000169)
phenotype: hek-293t cell (BTO:0002181)

Additional Notes

CRISPR GI screen
Cell Line:HEK293T EFO:0001184
Experimental Setup: Timecourse
GIST: A-phenotypic negative genetic interaction
Library: Dual-guide CRISPRn library
Significance Threshold:FDR ~ 0.3

Curated By

BioGRID

Interaction Summary

FNTA

Gene Ontology Biological Process

Gene Ontology Molecular Function

alpha-tubulin binding [IDA]microtubule binding [IDA]protein binding [IPI]protein farnesyltransferase activity [IDA]protein geranylgeranyltransferase activity [IDA]

Gene Ontology Cellular Component

TOP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]DNA topoisomerase type I activity [IDA, IMP]chromatin binding [IDA]core promoter sequence-specific DNA binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]