BAIT
CHEK1
CHK1
checkpoint kinase 1
GO Process (15)
GO Function (3)
GO Component (9)
Gene Ontology Biological Process
- DNA damage checkpoint [IDA, IMP]
- DNA damage induced protein phosphorylation [IDA]
- DNA repair [IMP]
- DNA replication [TAS]
- G2 DNA damage checkpoint [IMP]
- cellular response to DNA damage stimulus [IMP]
- cellular response to mechanical stimulus [IEP]
- chromatin-mediated maintenance of transcription [ISS]
- negative regulation of mitosis [IDA]
- peptidyl-threonine phosphorylation [IDA]
- regulation of double-strand break repair via homologous recombination [IDA]
- regulation of histone H3-K9 acetylation [ISS]
- regulation of mitotic centrosome separation [IDA]
- regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage [ISS]
- replicative senescence [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
IGF1R
CD221, IGFIR, IGFR, JTK13
insulin-like growth factor 1 receptor
GO Process (15)
GO Function (10)
GO Component (5)
Gene Ontology Biological Process
- immune response [IMP]
- inactivation of MAPKK activity [IDA]
- insulin receptor signaling pathway [TAS]
- insulin-like growth factor receptor signaling pathway [IDA]
- negative regulation of apoptotic process [IDA]
- peptidyl-tyrosine autophosphorylation [IMP]
- phosphatidylinositol 3-kinase signaling [IC]
- phosphatidylinositol-mediated signaling [IDA]
- positive regulation of DNA replication [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [TAS]
- protein autophosphorylation [IDA]
- protein tetramerization [IDA]
- regulation of JNK cascade [IDA]
- signal transduction [TAS]
Gene Ontology Molecular Function- identical protein binding [IPI]
- insulin binding [IPI]
- insulin receptor binding [IDA]
- insulin receptor substrate binding [IPI]
- insulin-like growth factor I binding [IPI]
- insulin-like growth factor binding [IDA]
- insulin-like growth factor-activated receptor activity [IDA]
- phosphatidylinositol 3-kinase binding [IPI]
- protein binding [IPI]
- protein tyrosine kinase activity [IDA, TAS]
- identical protein binding [IPI]
- insulin binding [IPI]
- insulin receptor binding [IDA]
- insulin receptor substrate binding [IPI]
- insulin-like growth factor I binding [IPI]
- insulin-like growth factor binding [IDA]
- insulin-like growth factor-activated receptor activity [IDA]
- phosphatidylinositol 3-kinase binding [IPI]
- protein binding [IPI]
- protein tyrosine kinase activity [IDA, TAS]
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions.
We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual guide RNAs in three cell lines, comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified, and these patterns replicated with combinatorial drugs at 75% precision. From these ... [more]
Nat. Methods Mar. 20, 2017; 0(); [Pubmed: 28319113]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- phenotype: viability (PATO:0000169)
- phenotype: hek-293t cell (BTO:0002181)
Additional Notes
- CRISPR GI screen
- Cell Line:HEK293T EFO:0001184
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Dual-guide CRISPRn library
- Significance Threshold:FDR ~ 0.3
Curated By
- BioGRID