BAIT
CDK4
CMM3, PSK-J3
cyclin-dependent kinase 4
GO Process (9)
GO Function (3)
GO Component (7)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IMP]
- mitotic cell cycle [TAS]
- negative regulation of cell cycle arrest [IDA]
- positive regulation of G2/M transition of mitotic cell cycle [IDA]
- positive regulation of cell proliferation [IMP]
- positive regulation of fibroblast proliferation [IMP]
- protein phosphorylation [IDA]
- regulation of gene expression [IMP]
- response to drug [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CHEK2
CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, RP11-436C9.1
checkpoint kinase 2
GO Process (17)
GO Function (6)
GO Component (4)
Gene Ontology Biological Process
- DNA damage checkpoint [TAS]
- DNA damage induced protein phosphorylation [IMP]
- G2/M transition of mitotic cell cycle [IMP]
- cellular protein catabolic process [IMP]
- cellular response to DNA damage stimulus [IMP, TAS]
- double-strand break repair [IMP]
- intrinsic apoptotic signaling pathway in response to DNA damage [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA]
- protein stabilization [IDA]
- regulation of protein catabolic process [IMP]
- regulation of transcription, DNA-templated [IDA]
- replicative senescence [NAS]
- signal transduction in response to DNA damage [IDA]
- signal transduction involved in intra-S DNA damage checkpoint [IMP]
- spindle assembly involved in mitosis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions.
We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual guide RNAs in three cell lines, comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified, and these patterns replicated with combinatorial drugs at 75% precision. From these ... [more]
Nat. Methods Mar. 20, 2017; 0(); [Pubmed: 28319113]
Throughput
- High Throughput
Ontology Terms
- phenotype: a-549 cell (BTO:0000018)
- phenotype: growth abnormality (HP:0001507)
- phenotype: viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: A-549 EFO:0001086
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Dual-guide CRISPRn library
- Significance Threshold:FDR ~ 0.3
Curated By
- BioGRID