BAIT

HDAC2

HD2, RPD3, YAF1
histone deacetylase 2
UBI
NEDD
SUMO
GO Process (29)
GO Function (13)
GO Component (8)

External Database Linkouts

CRISPR Database | HGNC | OMIM | Entrez Gene | RefSeq | UniprotKB | HPRD
Homo sapiens

PREY

MAP2K1

CFC3, MAPKK1, MEK1, MKK1, PRKMK1
mitogen-activated protein kinase kinase 1
UBI
SUMO
GO Process (36)
GO Function (6)
GO Component (11)

Gene Ontology Biological Process

External Database Linkouts

CRISPR Database | HGNC | OMIM | Entrez Gene | RefSeq | UniprotKB | HPRD
Homo sapiens

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions.

Shen JP, Zhao D, Sasik R, Luebeck J, Birmingham A, Bojorquez-Gomez A, Licon K, Klepper K, Pekin D, Beckett AN, Sanchez KS, Thomas A, Kuo CC, Du D, Roguev A, Lewis NE, Chang AN, Kreisberg JF, Krogan N, Qi L, Ideker T, Mali P

We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual guide RNAs in three cell lines, comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified, and these patterns replicated with combinatorial drugs at 75% precision. From these ... [more]

Nat. Methods Mar. 20, 2017; 0(); [Pubmed: 28319113]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: a-549 cell (BTO:0000018)
  • phenotype: growth abnormality (HP:0001507)
  • phenotype: viability (PATO:0000169)

Additional Notes

  • CRISPR GI screen
  • Cell Line: A-549 EFO:0001086
  • Experimental Setup: Timecourse
  • GIST: A-phenotypic negative genetic interaction
  • Library: Dual-guide CRISPRn library
  • Significance Threshold:FDR ~ 0.3

Curated By

  • BioGRID