BAIT

APBB1

FE65
amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
Rattus norvegicus
PREY

ENAH

ENA, MENA, NDPP1, RP11-496N12.7
enabled homolog (Drosophila)
GO Process (2)
GO Function (2)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

A single point mutation in a group I WW domain shifts its specificity to that of group II WW domains.

Espanel X, Sudol M

WW domains can be divided into three groups based on their binding specificity. By random mutagenesis, we switched the specificity of the Yes-associated protein (YAP) WW1 domain, a Group I WW domain, to that of the FE65 WW domain, which belongs to Group II. We showed that a single mutation, leucine 190 (betaB5) to tryptophan, is required to switch from ... [more]

J. Biol. Chem. Jun. 11, 1999; 274(24);17284-9 [Pubmed: 10358088]

Throughput

  • Low Throughput

Curated By

  • BioGRID