BAIT
SNCA
NACP, PARK1, PARK4, PD1
synuclein, alpha (non A4 component of amyloid precursor)
GO Process (45)
GO Function (20)
GO Component (16)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- calcium ion homeostasis [IDA]
- cellular response to copper ion [IDA]
- cellular response to epinephrine stimulus [TAS]
- cellular response to oxidative stress [IC]
- dopamine biosynthetic process [TAS]
- dopamine uptake involved in synaptic transmission [TAS]
- extracellular fibril organization [TAS]
- microglial cell activation [TAS]
- negative regulation of apoptotic process [IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of dopamine uptake involved in synaptic transmission [IDA]
- negative regulation of exocytosis [IMP]
- negative regulation of histone acetylation [IDA]
- negative regulation of microtubule polymerization [IDA]
- negative regulation of mitochondrial electron transport, NADH to ubiquinone [TAS]
- negative regulation of monooxygenase activity [IDA]
- negative regulation of norepinephrine uptake [IDA]
- negative regulation of platelet-derived growth factor receptor signaling pathway [IDA]
- negative regulation of serotonin uptake [IDA]
- negative regulation of thrombin receptor signaling pathway [IDA]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transporter activity [IDA]
- oxidation-reduction process [IDA]
- positive regulation of apoptotic process [TAS]
- positive regulation of endocytosis [IDA]
- positive regulation of glutathione peroxidase activity [IDA]
- positive regulation of hydrogen peroxide catabolic process [IDA]
- positive regulation of inositol phosphate biosynthetic process [IDA]
- positive regulation of peptidyl-serine phosphorylation [ISS]
- positive regulation of protein serine/threonine kinase activity [IDA]
- positive regulation of receptor recycling [IDA]
- positive regulation of release of sequestered calcium ion into cytosol [IDA]
- protein destabilization [IDA]
- receptor internalization [IDA]
- regulation of dopamine secretion [TAS]
- regulation of phospholipase activity [IDA]
- regulation of reactive oxygen species biosynthetic process [TAS]
- regulation of synaptic vesicle recycling [TAS]
- response to interferon-gamma [IDA]
- response to interleukin-1 [IDA]
- response to iron(II) ion [IDA]
- response to lipopolysaccharide [IDA]
- response to magnesium ion [IDA]
- synaptic vesicle endocytosis [ISS]
Gene Ontology Molecular Function- Hsp70 protein binding [IPI]
- alpha-tubulin binding [IPI]
- calcium ion binding [IDA]
- copper ion binding [IDA]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IDA]
- dynein binding [IPI]
- fatty acid binding [IDA]
- ferrous iron binding [IDA]
- histone binding [IDA]
- identical protein binding [IPI]
- kinesin binding [IPI]
- magnesium ion binding [IDA]
- oxidoreductase activity [IDA]
- phospholipase D inhibitor activity [IDA]
- phospholipid binding [IDA]
- phosphoprotein binding [IDA]
- protein binding [IPI]
- tau protein binding [IDA]
- transcription regulatory region DNA binding [TAS]
- zinc ion binding [IDA]
- Hsp70 protein binding [IPI]
- alpha-tubulin binding [IPI]
- calcium ion binding [IDA]
- copper ion binding [IDA]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IDA]
- dynein binding [IPI]
- fatty acid binding [IDA]
- ferrous iron binding [IDA]
- histone binding [IDA]
- identical protein binding [IPI]
- kinesin binding [IPI]
- magnesium ion binding [IDA]
- oxidoreductase activity [IDA]
- phospholipase D inhibitor activity [IDA]
- phospholipid binding [IDA]
- phosphoprotein binding [IDA]
- protein binding [IPI]
- tau protein binding [IDA]
- transcription regulatory region DNA binding [TAS]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
- actin cytoskeleton [IDA]
- axon [IDA]
- cell cortex [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- extracellular region [TAS]
- fibril [IDA]
- growth cone [IDA]
- inclusion body [IDA]
- lysosome [TAS]
- mitochondrial respiratory chain complex I [TAS]
- mitochondrion [TAS]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA]
- platelet alpha granule membrane [IDA]
Homo sapiens
PREY
SNCA
NACP, alphaSYN
synuclein, alpha
GO Process (66)
GO Function (24)
GO Component (25)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- adult locomotory behavior [IGI]
- behavioral response to cocaine [ISO]
- cellular response to copper ion [ISO]
- cellular response to oxidative stress [IMP]
- dopamine biosynthetic process [IMP]
- dopamine metabolic process [IGI]
- fatty acid metabolic process [IMP]
- long-term synaptic potentiation [IMP]
- membrane organization [IMP]
- microglial cell activation [IMP]
- mitochondrial ATP synthesis coupled electron transport [IMP]
- mitochondrial membrane organization [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of dopamine metabolic process [ISO]
- negative regulation of dopamine uptake involved in synaptic transmission [ISO]
- negative regulation of exocytosis [ISO]
- negative regulation of histone acetylation [ISO]
- negative regulation of microtubule polymerization [ISO]
- negative regulation of monooxygenase activity [ISO]
- negative regulation of neuron apoptotic process [IGI, ISO]
- negative regulation of norepinephrine uptake [ISO]
- negative regulation of platelet-derived growth factor receptor signaling pathway [ISO]
- negative regulation of protein phosphorylation [ISO]
- negative regulation of serotonin uptake [ISO]
- negative regulation of thrombin receptor signaling pathway [ISO]
- negative regulation of transporter activity [ISO]
- neutral lipid metabolic process [IMP]
- oxidation-reduction process [ISO]
- phospholipid metabolic process [IMP]
- positive regulation of endocytosis [ISO]
- positive regulation of glutathione peroxidase activity [ISO]
- positive regulation of hydrogen peroxide catabolic process [ISO]
- positive regulation of inositol phosphate biosynthetic process [ISO]
- positive regulation of neurotransmitter secretion [IDA]
- positive regulation of peptidyl-serine phosphorylation [IMP]
- positive regulation of protein serine/threonine kinase activity [ISO]
- positive regulation of receptor recycling [ISO]
- positive regulation of release of sequestered calcium ion into cytosol [ISO]
- positive regulation of synaptic transmission [IMP]
- protein destabilization [ISO]
- receptor internalization [ISO]
- regulation of acyl-CoA biosynthetic process [IDA, IMP]
- regulation of dopamine secretion [IGI]
- regulation of excitatory postsynaptic membrane potential [IMP]
- regulation of glutamate secretion [IMP]
- regulation of locomotion [IMP]
- regulation of long-term neuronal synaptic plasticity [IMP]
- regulation of macrophage activation [IMP]
- regulation of neuron apoptotic process [IMP]
- regulation of neuron death [ISO]
- regulation of neuronal synaptic plasticity [IMP]
- regulation of neurotransmitter secretion [IGI, IMP]
- regulation of phospholipase activity [ISO]
- response to drug [IMP]
- response to interferon-gamma [ISO]
- response to interleukin-1 [ISO]
- response to iron(II) ion [ISO]
- response to lipopolysaccharide [ISO]
- response to magnesium ion [ISO]
- synapse organization [IGI]
- synaptic transmission [IGI]
- synaptic transmission, dopaminergic [IMP]
- synaptic vesicle endocytosis [IMP]
- synaptic vesicle transport [IMP]
Gene Ontology Molecular Function- Hsp70 protein binding [ISO]
- alpha-tubulin binding [ISO]
- arachidonic acid binding [IDA]
- beta-tubulin binding [ISO]
- calcium ion binding [ISO]
- copper ion binding [ISO]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [ISO]
- dynein binding [ISO]
- enzyme binding [ISO]
- ferrous iron binding [ISO]
- histone binding [IDA, ISO]
- identical protein binding [IDA, ISO]
- kinesin binding [ISO]
- magnesium ion binding [ISO]
- microtubule binding [ISO]
- oxidoreductase activity [ISO]
- phospholipase binding [ISO]
- phospholipid binding [ISO]
- phosphoprotein binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- tau protein binding [ISO]
- zinc ion binding [ISO]
- Hsp70 protein binding [ISO]
- alpha-tubulin binding [ISO]
- arachidonic acid binding [IDA]
- beta-tubulin binding [ISO]
- calcium ion binding [ISO]
- copper ion binding [ISO]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [ISO]
- dynein binding [ISO]
- enzyme binding [ISO]
- ferrous iron binding [ISO]
- histone binding [IDA, ISO]
- identical protein binding [IDA, ISO]
- kinesin binding [ISO]
- magnesium ion binding [ISO]
- microtubule binding [ISO]
- oxidoreductase activity [ISO]
- phospholipase binding [ISO]
- phospholipid binding [ISO]
- phosphoprotein binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- tau protein binding [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- actin cytoskeleton [ISO]
- axon [ISO]
- axon terminus [ISO]
- cell [IMP]
- cell cortex [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic vesicle membrane [ISO]
- cytoskeleton [IDA]
- cytosol [IDA, ISO]
- fibril [ISO]
- growth cone [ISO]
- inclusion body [IDA, ISO]
- intracellular membrane-bounded organelle [ISO]
- mitochondrion [IMP, ISO]
- nuclear outer membrane [ISO]
- nucleus [IDA, ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- platelet alpha granule membrane [ISO]
- ribosome [ISO]
- rough endoplasmic reticulum [ISO]
- synapse [IDA]
- synaptic vesicle [ISO]
- terminal bouton [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Induction of de novo α-synuclein fibrillization in a neuronal model for Parkinson's disease.
Lewy bodies (LBs) are intraneuronal inclusions consisting primarily of fibrillized human α-synuclein (hα-Syn) protein, which represent the major pathological hallmark of Parkinson's disease (PD). Although doubling hα-Syn expression provokes LB pathology in humans, hα-Syn overexpression does not trigger the formation of fibrillar LB-like inclusions in mice. We hypothesized that interactions between exogenous hα-Syn and endogenous mouse synuclein homologs could be ... [more]
Proc. Natl. Acad. Sci. U.S.A. Feb. 16, 2016; 113(7);E912-21 [Pubmed: 26839406]
Throughput
- Low Throughput
Curated By
- BioGRID