USP50
PYCARD
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [NAS]
- activation of innate immune response [IDA]
- apoptotic process [TAS]
- cellular response to interleukin-1 [IDA]
- cellular response to lipopolysaccharide [IDA]
- cellular response to tumor necrosis factor [IDA]
- defense response to Gram-negative bacterium [IMP]
- defense response to virus [IDA]
- innate immune response [TAS]
- intrinsic apoptotic signaling pathway by p53 class mediator [IMP]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- macropinocytosis [ISS]
- myeloid dendritic cell activation [IMP]
- myeloid dendritic cell activation involved in immune response [ISS]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- negative regulation of NF-kappaB transcription factor activity [IMP]
- negative regulation of interferon-beta production [IMP]
- negative regulation of protein serine/threonine kinase activity [IDA]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of JNK cascade [IMP]
- positive regulation of NF-kappaB transcription factor activity [IDA]
- positive regulation of T cell activation [IMP]
- positive regulation of T cell migration [ISS]
- positive regulation of actin filament polymerization [ISS]
- positive regulation of activated T cell proliferation [ISS]
- positive regulation of adaptive immune response [IMP]
- positive regulation of antigen processing and presentation of peptide antigen via MHC class II [ISS]
- positive regulation of apoptotic process [IDA]
- positive regulation of chemokine secretion [IMP]
- positive regulation of cysteine-type endopeptidase activity [IDA]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- positive regulation of extrinsic apoptotic signaling pathway [IDA]
- positive regulation of interferon-gamma production [ISS]
- positive regulation of interleukin-1 beta secretion [IDA]
- positive regulation of interleukin-10 secretion [IMP]
- positive regulation of interleukin-6 production [ISS]
- positive regulation of interleukin-6 secretion [IMP]
- positive regulation of interleukin-8 secretion [IMP]
- positive regulation of phagocytosis [ISS]
- positive regulation of release of cytochrome c from mitochondria [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of tumor necrosis factor production [IMP]
- regulation of intrinsic apoptotic signaling pathway [IDA]
- regulation of protein stability [ISS]
- regulation of tumor necrosis factor-mediated signaling pathway [IMP]
- signal transduction [NAS]
- tumor necrosis factor-mediated signaling pathway [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The deubiquitinating enzyme, ubiquitin-specific peptidase 50, regulates inflammasome activation by targeting the ASC adaptor protein.
NOD-like receptor family protein 3 (NLRP3)-mediated inflammasome activation promotes caspase-1-dependent production of interleukin-1β (IL-1β) and requires the adaptor protein ASC. Compared with the priming and activation mechanisms of the inflammasome signaling pathway, post-translational ubiquitination/deubiquitination mechanisms controlling inflammasome activation have not been clearly addressed. We here demonstrate that the deubiquitinating enzyme USP50 binds to the ASC protein and subsequently regulates the ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| USP50 PYCARD | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 2209244 |
Curated By
- BioGRID