Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Expression of myristoyltransferase and its interacting proteins in epilepsy.

Selvakumar P, Lakshmikuttyamma A, Charavaryamath C, Singh B, Tuchek J, Sharma RK

N-Myristoylation is a co-translational, irreversible addition of a fatty acyl moiety to the amino terminus of many eukaryotic cellular proteins. This modification is catalyzed by N-myristoyltransferase (NMT) and is recognized to be a widespread and functionally important modification of proteins. The myristoylated Src family kinases are involved in various signaling cascades, including the N-methyl-d-aspartate receptor functions. We examined the expression ... [more]

Biochem. Biophys. Res. Commun. Oct. 07, 2005; 335(4);1132-9 [Pubmed: 16129091]

Throughput

  • Low Throughput

Curated By

  • BioGRID