Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Schizophrenia susceptibility gene product dysbindin-1 regulates the homeostasis of cyclin D1.

Ito H, Morishita R, Nagata K

Dysbindin-1 (dystrobrevin binding protein-1, DTNBP1) is now widely accepted as a potential schizophrenia susceptibility gene and accumulating evidence indicates its functions in the neural development. In this study, we tried to identify new binding partners for dysbindin-1 to clarify the novel function of this molecule. When consulted with BioGRID protein interaction database, cyclin D3 was found to be a possible ... [more]

Biochim. Biophys. Acta Aug. 01, 2016; 1862(8);1383-91 [Pubmed: 27130439]

Throughput

  • Low Throughput

Additional Notes

  • DTNBP1 isoform Q96EV8-1

Curated By

  • BioGRID