SIX1
Gene Ontology Biological Process
- branching involved in ureteric bud morphogenesis [ISS]
- embryonic cranial skeleton morphogenesis [ISS]
- embryonic skeletal system morphogenesis [ISS]
- epithelial cell differentiation [ISS]
- generation of neurons [ISS]
- inner ear development [ISS]
- inner ear morphogenesis [ISS]
- kidney development [ISS]
- mesonephric tubule formation [ISS]
- metanephric mesenchyme development [ISS]
- myoblast migration [ISS]
- negative regulation of neuron apoptotic process [ISS]
- organ induction [ISS]
- pattern specification process [ISS]
- positive regulation of branching involved in ureteric bud morphogenesis [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [ISS]
- positive regulation of ureteric bud formation [ISS]
- protein localization to nucleus [IDA]
- regulation of branch elongation involved in ureteric bud branching [ISS]
- regulation of neuron differentiation [ISS]
- regulation of transcription, DNA-templated [ISS]
- skeletal muscle tissue development [ISS]
- thymus development [ISS]
- thyroid gland development [ISS]
- ureteric bud development [ISS]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISS]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISS]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
EYA1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Architecture of the human interactome defines protein communities and disease networks.
The physiology of a cell can be viewed as the product of thousands of proteins acting in concert to shape the cellular response. Coordination is achieved in part through networks of protein-protein interactions that assemble functionally related proteins into complexes, organelles, and signal transduction pathways. Understanding the architecture of the human proteome has the potential to inform cellular, structural, and ... [more]
Quantitative Score
- 1.0 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 2.0 HEK 293T cells CompPASS score = 1.0, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 3.0 (PMID: 33961781) and may be re-scored from BioPlex 1.0 (PMID: 26186194). Only scores from within BioPlex 2.0 (PMID: 28514442) should be compared directly.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SIX1 EYA1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 1180342 | |
| SIX1 EYA1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3062387 | |
| EYA1 SIX1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| EYA1 SIX1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| EYA1 SIX1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2708608 |
Curated By
- BioGRID