An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Architecture of the human interactome defines protein communities and disease networks.

Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW

The physiology of a cell can be viewed as the product of thousands of proteins acting in concert to shape the cellular response. Coordination is achieved in part through networks of protein-protein interactions that assemble functionally related proteins into complexes, organelles, and signal transduction pathways. Understanding the architecture of the human proteome has the potential to inform cellular, structural, and ... [more]

Nature May. 25, 2017; 545(7655);505-509 [Pubmed: 28514442]

Quantitative Score

0.999999964 [compPASS Score]

Throughput

High Throughput

Additional Notes

BioPlex 2.0 HEK 293T cells CompPASS score = 0.999999964, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
This data has also been reanalyzed as part of BioPlex 3.0 (PMID: 33961781) and may be re-scored from BioPlex 1.0 (PMID: 26186194). Only scores from within BioPlex 2.0 (PMID: 28514442) should be compared directly.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
XRCC1 POLB	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hein MY (2015)	High	-	BioGRID	1446185
POLB XRCC1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2015)	High	1	BioGRID	1188355
POLB XRCC1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	1	BioGRID	3041973
XRCC1 POLB	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Liu X (2021)	Low	-	BioGRID	-
POLB XRCC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Bhattacharyya N (2001)	Low	-	BioGRID	-
POLB XRCC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Fang Q (2014)	Low	-	BioGRID	-
XRCC1 POLB	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Fang Q (2014)	Low	-	BioGRID	-
XRCC1 POLB	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Hu LY (2018)	Low	-	BioGRID	-
XRCC1 POLB	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Liu X (2021)	Low	-	BioGRID	-
XRCC1 POLB	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Luo H (2004)	Low	-	BioGRID	-
XRCC1 POLB	Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe.	Hu LY (2018)	Low	-	BioGRID	-
XRCC1 POLB	Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe.	Kubota Y (1996)	Low	-	BioGRID	-
POLB XRCC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Bhattacharyya N (2001)	Low	-	BioGRID	-
XRCC1 POLB	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Fan J (2004)	Low	-	BioGRID	-
XRCC1 POLB	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hu LY (2018)	Low	-	BioGRID	-
POLB XRCC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hu LY (2018)	Low	-	BioGRID	-
XRCC1 POLB	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Kubota Y (1996)	Low	-	BioGRID	-
XRCC1 POLB	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Taylor RM (2002)	Low	-	BioGRID	-
POLB XRCC1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Wang L (2004)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

POLB

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA-directed DNA polymerase activity [IDA]enzyme binding [IPI]lyase activity [IDA]microtubule binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

XRCC1