Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.


Specific association of estrogen receptor beta with the cell cycle spindle assembly checkpoint protein, MAD2.

Poelzl G, Kasai Y, Mochizuki N, Shaul PW, Brown M, Mendelsohn ME

Estrogen receptors (ERs) are ligand-activated transcription factors that regulate gene expression and cell growth. Two ERs now have been identified: ERalpha and the more recently discovered ERbeta. The physiological function of ERbeta remains unclear, but evidence from vascular injury studies and from ERbeta knockout mice suggests that ERbeta may be involved in the regulation of cellular proliferation. Here we show ... [more]

Proc. Natl. Acad. Sci. U.S.A. Mar. 14, 2000; 97(6);2836-9 [Pubmed: 10706629]


  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.


Curated By

  • BioGRID