VAMP4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
VTI1B
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [IBA]
- Golgi to vacuole transport [IBA]
- autophagic vacuole fusion [IMP]
- cell proliferation [TAS]
- intra-Golgi vesicle-mediated transport [IBA]
- membrane fusion [TAS]
- protein targeting to vacuole [IBA]
- regulation of protein localization to plasma membrane [IDA]
- retrograde transport, endosome to Golgi [IBA]
- vesicle docking involved in exocytosis [TAS]
- vesicle fusion with Golgi apparatus [IBA]
- vesicle-mediated transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- ER to Golgi transport vesicle membrane [IBA]
- Golgi apparatus [IDA]
- SNARE complex [IBA]
- cytoplasm [IDA]
- endoplasmic reticulum membrane [IBA]
- intracellular membrane-bounded organelle [IDA]
- late endosome membrane [IDA]
- lysosomal membrane [IDA]
- neuronal cell body [ISS]
- perinuclear region of cytoplasm [IDA]
- recycling endosome [IDA]
- synaptic vesicle [ISS]
- vesicle [IDA]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Architecture of the human interactome defines protein communities and disease networks.
The physiology of a cell can be viewed as the product of thousands of proteins acting in concert to shape the cellular response. Coordination is achieved in part through networks of protein-protein interactions that assemble functionally related proteins into complexes, organelles, and signal transduction pathways. Understanding the architecture of the human proteome has the potential to inform cellular, structural, and ... [more]
Quantitative Score
- 0.970141255 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 2.0 HEK 293T cells CompPASS score = 0.970141255, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 3.0 (PMID: 33961781) and may be re-scored from BioPlex 1.0 (PMID: 26186194). Only scores from within BioPlex 2.0 (PMID: 28514442) should be compared directly.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| VTI1B VAMP4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3375137 | |
| VAMP4 VTI1B | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9776 | BioGRID | 3042272 | |
| VAMP4 VTI1B | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID