BAIT

LEO1

L000000936, YOR123C

Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay

GO Process (8)

GO Function (3)

GO Component (2)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RTF1

CSL3, L000001782, YGL244W

Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay

GO Process (19)

GO Function (3)

GO Component (3)

Gene Ontology Biological Process

DNA-templated transcription, termination [IMP]

global genome nucleotide-excision repair [IMP]

mRNA 3'-end processing [IMP]

negative regulation of transcription from RNA polymerase II promoter [IMP]

positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]

positive regulation of transcription elongation from RNA polymerase I promoter [IDA]

positive regulation of transcription elongation from RNA polymerase II promoter [IMP]

recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [IMP]

regulation of chromatin silencing at telomere [IMP]

regulation of histone H2B conserved C-terminal lysine ubiquitination [IDA, IMP]

regulation of histone H2B ubiquitination [IMP]

regulation of histone H3-K4 methylation [IMP]

regulation of histone H3-K79 methylation [IMP]

regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]

regulation of transcription from RNA polymerase II promoter [IGI]

regulation of transcription-coupled nucleotide-excision repair [IGI]

snoRNA 3'-end processing [IMP]

snoRNA transcription from an RNA polymerase II promoter [IMP]

transcription elongation from RNA polymerase II promoter [IGI, IMP]

Gene Ontology Molecular Function

RNA binding [IDA]
RNA polymerase II C-terminal domain phosphoserine binding [IDA]
RNA polymerase II transcription factor binding transcription factor activity [IPI]

Gene Ontology Cellular Component

Cdc73/Paf1 complex [IPI]

nucleus [IDA]

transcriptionally active chromatin [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Functional dissection of protein complexes involved in yeast chromosome biology using a genetic interaction map.

Collins SR, Miller KM, Maas NL, Roguev A, Fillingham J, Chu CS, Schuldiner M, Gebbia M, Recht J, Shales M, Ding H, Xu H, Han J, Ingvarsdottir K, Cheng B, Andrews B, Boone C, Berger SL, Hieter P, Zhang Z, Brown GW, Ingles CJ, Emili A, Allis CD, Toczyski DP, Weissman JS, Greenblatt JF, Krogan NJ

Defining the functional relationships between proteins is critical for understanding virtually all aspects of cell biology. Large-scale identification of protein complexes has provided one important step towards this goal; however, even knowledge of the stoichiometry, affinity and lifetime of every protein-protein interaction would not reveal the functional relationships between and within such complexes. Genetic interactions can provide functional information that ... [more]

Nature Apr. 12, 2007; 446(7137);806-10 [Pubmed: 17314980]

Quantitative Score

-2.532428 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

An Epistatic MiniArray Profile (E-MAP) analysis was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.5 for positive interactions (suppression) and S score < -2.5 for negative interactions (synthetic sick/lethality).

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RTF1 LEO1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2002)	High	-	BioGRID	-
RTF1 LEO1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
LEO1 RTF1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2002)	Low	-	BioGRID	-
RTF1 LEO1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2002)	Low	-	BioGRID	-
LEO1 RTF1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2004)	High	-	BioGRID	-
RTF1 LEO1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2004)	High	-	BioGRID	-
RTF1 LEO1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
RTF1 LEO1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Mayekar MK (2013)	Low	-	BioGRID	-
RTF1 LEO1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3614454
LEO1 RTF1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Nordick K (2008)	Low	-	BioGRID	-
LEO1 RTF1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Squazzo SL (2002)	Low	-	BioGRID	-
RTF1 LEO1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Squazzo SL (2002)	Low	-	BioGRID	-
RTF1 LEO1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Xu Y (2017)	Low	-	BioGRID	2334931

Curated By

BioGRID

Interaction Summary

LEO1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [IDA]RNA polymerase II C-terminal domain phosphoserine binding [IDA]RNA polymerase II transcription factor binding transcription factor activity [IPI]

Gene Ontology Cellular Component

RTF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [IDA]RNA polymerase II C-terminal domain phosphoserine binding [IDA]RNA polymerase II transcription factor binding transcription factor activity [IPI]

Gene Ontology Cellular Component

Negative Genetic

Publication

Functional dissection of protein complexes involved in yeast chromosome biology using a genetic interaction map.

Quantitative Score

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

RNA binding [IDA]
RNA polymerase II C-terminal domain phosphoserine binding [IDA]
RNA polymerase II transcription factor binding transcription factor activity [IPI]

RNA binding [IDA]
RNA polymerase II C-terminal domain phosphoserine binding [IDA]
RNA polymerase II transcription factor binding transcription factor activity [IPI]