Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The cerebellar leucine-rich acidic nuclear protein interacts with ataxin-1.

Matilla A, Koshy BT, Cummings CJ, Isobe T, Orr HT, Zoghbi HY

Spinocerebellar ataxia type 1 (SCA1) is an autosomal dominant neurodegenerative disorder characterized by ataxia, progressive motor deterioration, and loss of cerebellar Purkinje cells. SCA1 belongs to a growing group of neurodegenerative disorders caused by expansion of CAG repeats, which encode glutamine. Although the proteins containing these repeats are widely expressed, the neurodegeneration in SCA1 and other polyglutamine diseases selectively involves ... [more]

Nature Oct. 30, 1997; 389(6654);974-8 [Pubmed: 9353121]

Throughput

  • Low Throughput

Curated By

  • BioGRID