TRS20
Gene Ontology Biological Process
Gene Ontology Cellular Component
TRS20
Gene Ontology Biological Process
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
mBET3 is required for the organization of the TRAPP complexes.
Large tethering complexes mediate the initial interaction of a transport vesicle with its target membrane. There are two forms of the multi-subunit tethering complex called TRAPP (TRAPPI and TRAPPII) that tether transport vesicles in different trafficking steps. Understanding TRAPP complex assembly and the protein-protein interactions among the subunits is an important step in elucidating the function of this tether. Here ... [more]
Throughput
- Low Throughput
Additional Notes
- weak interaction
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
TRS20 TRS20 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
TRS20 TRS20 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 3310563 |
Curated By
- BioGRID