BAIT

TRAPPC3

1110058K12Rik, AI835942, AU019197, Bet3, RP23-118D1.2
trafficking protein particle complex 3
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

TRAPPC1

BET5, D11Ertd172e, MUM2, RP23-26L6.10-004
trafficking protein particle complex 1
GO Process (2)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

mBET3 is required for the organization of the TRAPP complexes.

Menon S, Cai H, Lu H, Dong G, Cai Y, Reinisch K, Ferro-Novick S

Large tethering complexes mediate the initial interaction of a transport vesicle with its target membrane. There are two forms of the multi-subunit tethering complex called TRAPP (TRAPPI and TRAPPII) that tether transport vesicles in different trafficking steps. Understanding TRAPP complex assembly and the protein-protein interactions among the subunits is an important step in elucidating the function of this tether. Here ... [more]

Biochem. Biophys. Res. Commun. Nov. 24, 2006; 350(3);669-77 [Pubmed: 17027922]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TRAPPC3 TRAPPC1
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.988BioGRID
2676257
TRAPPC3 TRAPPC1
Co-purification
Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Low-BioGRID
-

Curated By

  • BioGRID