Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The BioPlex Network of Human Protein Interactions: Additional Unpublished AP-MS Results

Huttlin EL, Ting L, Bruckner RJ, Paulo JA, Gygi MP, Rad R, Kolippakkam D, Szpyt J, Zarraga G, Tam S, Gebreab F, Colby G, Pontano-Vaites L, Obar RA, Guarani-Pereira V, Harris T, Artavanis-Tsakonas S, Sowa ME, Harper JW, Gygi SP

As part of an ongoing collaboration between the labs of Steve Gygi and Wade Harper in the Department of Cell Biology at Harvard Medical School, we are profiling systematically the interactions among human proteins using affinity purification mass spectrometry. HA-tagged baits taken from the human ORFeome (version 8.1; Marc Vidal) have been expressed individually in HEK 293T cells, followed by ... [more]

Status: Pre-Publication Dataset

Quantitative Score

  • 0.999587644 [compPASS Score]

Throughput

  • High Throughput

Additional Notes

  • Additional Unpublished AP-MS Results from the BioPlex project, http://bioplex.hms.harvard.edu/. Unpublished interactions are not available directly for download. Please contact Ed Huttlin (edward_huttlin@hms.harvard.edu), Steve Gygi (steven_gygi@hms.harvard.edu), or Wade Harper (wade_harper@hms.harvard.edu) for further information.
  • Quantitative scores are a modified CompPASS score derived from Sowa et al., Cell, 2009 (PMID 19615732). The cut-off threshold is > 0.75.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LSM2 LSM7
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.996BioGRID
741203
LSM2 LSM7
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High1BioGRID
1264233
LSM2 LSM7
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2814736
LSM2 LSM7
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
LSM7 LSM2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
LSM2 LSM7
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
LSM2 LSM7
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
LSM7 LSM2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
LSM7 LSM2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2722331

Curated By

  • BioGRID