BAIT

HRAS

C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
Harvey rat sarcoma viral oncogene homolog
GO Process (39)
GO Function (3)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The BioPlex Network of Human Protein Interactions: Additional Unpublished AP-MS Results

Huttlin EL, Ting L, Bruckner RJ, Paulo JA, Gygi MP, Rad R, Kolippakkam D, Szpyt J, Zarraga G, Tam S, Gebreab F, Colby G, Pontano-Vaites L, Obar RA, Guarani-Pereira V, Harris T, Artavanis-Tsakonas S, Sowa ME, Harper JW, Gygi SP

As part of an ongoing collaboration between the labs of Steve Gygi and Wade Harper in the Department of Cell Biology at Harvard Medical School, we are profiling systematically the interactions among human proteins using affinity purification mass spectrometry. HA-tagged baits taken from the human ORFeome (version 8.1; Marc Vidal) have been expressed individually in HEK 293T cells, followed by ... [more]

Status: Pre-Publication Dataset

Quantitative Score

  • 0.997423293 [compPASS Score]

Throughput

  • High Throughput

Additional Notes

  • Additional Unpublished AP-MS Results from the BioPlex project, http://bioplex.hms.harvard.edu/. Unpublished interactions are not available directly for download. Please contact Ed Huttlin (edward_huttlin@hms.harvard.edu), Steve Gygi (steven_gygi@hms.harvard.edu), or Wade Harper (wade_harper@hms.harvard.edu) for further information.
  • Quantitative scores are a modified CompPASS score derived from Sowa et al., Cell, 2009 (PMID 19615732). The cut-off threshold is > 0.75.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HRAS RAP1GDS1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
RAP1GDS1 HRAS
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
RAP1GDS1 HRAS
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2726810
RAP1GDS1 HRAS
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID