An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Defining the Protein-Protein Interaction Network of the Human Protein Tyrosine Phosphatase Family.

Li X, Tran KM, Aziz KE, Sorokin AV, Chen J, Wang W

Protein tyrosine phosphorylation, which plays a vital role in a variety of human cellular processes, is coordinated by protein tyrosine kinases and protein tyrosine phosphatases (PTPs). Genomic studies provide compelling evidence that PTPs are frequently mutated in various human cancers, suggesting that they have important roles in tumor suppression. However, the cellular functions and regulatory machineries of most PTPs are ... [more]

Mol. Cell Proteomics Sep. 01, 2016; 15(9);3030-44 [Pubmed: 27432908]

Throughput

High Throughput

Additional Notes

high-confidence candidate interacting protein

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RNGTT RNGTT	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Horlbeck MA (2018)	High	-7.6047	BioGRID	2458668

Curated By

BioGRID

Interaction Summary

RNGTT

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA guanylyltransferase activity [IDA]mRNA guanylyltransferase activity [IDA]triphosphatase activity [IDA]

Gene Ontology Cellular Component