Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.


Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras.

Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander ES, Sabatini DM

The genetic dependencies of human cancers widely vary. Here, we catalog this heterogeneity and use it to identify functional gene interactions and genotype-dependent liabilities in cancer. By using genome-wide CRISPR-based screens, we generate a gene essentiality dataset across 14 human acute myeloid leukemia (AML) cell lines. Sets of genes with correlated patterns of essentiality across the lines reveal new gene ... [more]

Cell Feb. 23, 2017; 168(5);890-903.e15 [Pubmed: 28162770]


  • High Throughput

Ontology Terms

  • phenotype: growth abnormality (HP:0001507)
  • phenotype: viability (PATO:0000169)

Additional Notes

  • Hit genes show differential essentiality in Ras oncogenic mutant cell lines (KRAS and NRAS)
  • Negative genetic

Curated By

  • BioGRID