UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
IKBKB
Gene Ontology Biological Process
- B cell homeostasis [IMP]
- I-kappaB phosphorylation [IDA, TAS]
- cellular response to tumor necrosis factor [ISO]
- neuron projection development [ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of cation channel activity [IGI]
- positive regulation of cell proliferation [ISO]
- positive regulation of neuron death [ISO]
- positive regulation of sodium ion transport [IGI]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- protein phosphorylation [IMP, ISO]
- serine phosphorylation of STAT protein [ISO]
- signal transduction by phosphorylation [TAS]
Gene Ontology Molecular Function- ATP binding [ISO]
- IkappaB kinase activity [IDA]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- protein kinase activity [IDA, IMP, ISO]
- protein kinase binding [ISO]
- receptor signaling protein serine/threonine kinase activity [TAS]
- scaffold protein binding [ISO]
- ATP binding [ISO]
- IkappaB kinase activity [IDA]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- protein kinase activity [IDA, IMP, ISO]
- protein kinase binding [ISO]
- receptor signaling protein serine/threonine kinase activity [TAS]
- scaffold protein binding [ISO]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
An Interaction Landscape of Ubiquitin Signaling.
Intracellular signaling via the covalent attachment of different ubiquitin linkages to protein substrates is fundamental to many cellular processes. Although linkage-selective ubiquitin interactors have been studied on a case-by-case basis, proteome-wide analyses have not been conducted yet. Here, we present ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS), a quantitative interaction proteomics method that makes use of chemically synthesized diubiquitin to enrich ... [more]
Throughput
- High Throughput
Additional Notes
- #LPPI
- Chemically synthesized diubiquitin
- Likely protein-protein interaction
- Mouse embryonic stem cells (ESCs)
- Ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS)
Curated By
- BioGRID