UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
BRCA1
Gene Ontology Biological Process
- DNA replication [IMP]
- G2 DNA damage checkpoint [ISO]
- cellular response to DNA damage stimulus [IMP]
- cellular response to indole-3-methanol [ISO]
- cellular response to tumor necrosis factor [ISO]
- centrosome cycle [IGI]
- centrosome duplication [TAS]
- chordate embryonic development [IMP]
- chromosome segregation [ISO]
- dosage compensation by inactivation of X chromosome [IDA]
- double-strand break repair [ISO]
- double-strand break repair via homologous recombination [IBA, ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage [ISO]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [ISO]
- negative regulation of fatty acid biosynthetic process [IBA, ISO]
- negative regulation of histone H3-K4 methylation [IMP]
- negative regulation of histone H3-K9 methylation [IMP, ISO]
- negative regulation of histone acetylation [IMP]
- negative regulation of reactive oxygen species metabolic process [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of DNA repair [ISO]
- positive regulation of angiogenesis [ISO]
- positive regulation of cell cycle arrest [IBA, ISO]
- positive regulation of gene expression [ISO]
- positive regulation of histone H3-K4 methylation [IMP, ISO]
- positive regulation of histone H3-K9 acetylation [IMP, ISO]
- positive regulation of histone H3-K9 methylation [IMP]
- positive regulation of histone H4-K16 acetylation [IMP, ISO]
- positive regulation of histone H4-K20 methylation [IMP, ISO]
- positive regulation of histone acetylation [IMP, ISO]
- positive regulation of protein ubiquitination [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- positive regulation vascular endothelial growth factor production [ISO]
- postreplication repair [ISO]
- protein K6-linked ubiquitination [ISO]
- protein autoubiquitination [ISO]
- protein ubiquitination [IBA, ISO]
- regulation of DNA methylation [IMP]
- regulation of cell motility [IBA]
- regulation of cell proliferation [IBA]
- regulation of gene expression by genetic imprinting [IMP]
- response to estrogen [ISO]
- response to ionizing radiation [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
An Interaction Landscape of Ubiquitin Signaling.
Intracellular signaling via the covalent attachment of different ubiquitin linkages to protein substrates is fundamental to many cellular processes. Although linkage-selective ubiquitin interactors have been studied on a case-by-case basis, proteome-wide analyses have not been conducted yet. Here, we present ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS), a quantitative interaction proteomics method that makes use of chemically synthesized diubiquitin to enrich ... [more]
Throughput
- High Throughput
Additional Notes
- #LPPI
- Chemically synthesized diubiquitin
- Likely protein-protein interaction
- Mouse embryonic stem cells (ESCs)
- Ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS)
Curated By
- BioGRID