UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PML
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [IMP]
- PML body organization [IDA, ISO]
- SMAD protein import into nucleus [IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- apoptotic process [ISO]
- branching involved in mammary gland duct morphogenesis [IMP]
- cell aging [IMP]
- cell cycle arrest [IMP, ISO]
- cell fate commitment [IMP]
- cellular response to interleukin-4 [IDA]
- cellular senescence [ISO]
- circadian regulation of gene expression [IDA]
- common-partner SMAD protein phosphorylation [IMP]
- endoplasmic reticulum calcium ion homeostasis [IMP]
- entrainment of circadian clock by photoperiod [IDA]
- extrinsic apoptotic signaling pathway [IMP]
- innate immune response [IDA, ISO]
- intrinsic apoptotic signaling pathway by p53 class mediator [IMP]
- intrinsic apoptotic signaling pathway in response to DNA damage [IMP, ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [IMP]
- intrinsic apoptotic signaling pathway in response to oxidative stress [IMP]
- maintenance of protein location in nucleus [ISO]
- myeloid cell differentiation [IMP]
- negative regulation of angiogenesis [IMP, ISO]
- negative regulation of cell growth [ISO]
- negative regulation of cell proliferation [IMP, ISO]
- negative regulation of mitotic cell cycle [ISO]
- negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO]
- negative regulation of telomerase activity [ISO]
- negative regulation of telomere maintenance via telomerase [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- negative regulation of translation in response to oxidative stress [ISO]
- negative regulation of viral release from host cell [IDA, ISO]
- positive regulation of apoptotic process involved in mammary gland involution [ISO]
- positive regulation of apoptotic signaling pathway [IGI, IMP]
- positive regulation of defense response to virus by host [ISO]
- positive regulation of extrinsic apoptotic signaling pathway [ISO]
- positive regulation of histone deacetylation [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein complex assembly [ISO]
- protein stabilization [ISO]
- protein targeting [ISO]
- regulation of MHC class I biosynthetic process [IDA]
- regulation of calcium ion transport into cytosol [IMP]
- regulation of circadian rhythm [IDA]
- regulation of double-strand break repair [ISO]
- regulation of nucleic acid-templated transcription [TAS]
- regulation of protein phosphorylation [IMP]
- regulation of transcription, DNA-templated [IDA, ISO]
- response to UV [IMP]
- response to cytokine [ISO]
- response to gamma radiation [IMP]
- response to hypoxia [IMP, ISO]
- retinoic acid receptor signaling pathway [IMP]
- transforming growth factor beta receptor signaling pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
An Interaction Landscape of Ubiquitin Signaling.
Intracellular signaling via the covalent attachment of different ubiquitin linkages to protein substrates is fundamental to many cellular processes. Although linkage-selective ubiquitin interactors have been studied on a case-by-case basis, proteome-wide analyses have not been conducted yet. Here, we present ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS), a quantitative interaction proteomics method that makes use of chemically synthesized diubiquitin to enrich ... [more]
Throughput
- High Throughput
Additional Notes
- #LPPI
- Chemically synthesized diubiquitin
- Likely protein-protein interaction
- Mouse embryonic stem cells (ESCs)
- Ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS)
Curated By
- BioGRID