UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ERCC2
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- DNA duplex unwinding [ISO]
- DNA repair [IMP]
- UV protection [IMP, ISO]
- aging [IMP]
- apoptotic process [ISO]
- bone mineralization [IMP]
- cell proliferation [IMP]
- central nervous system myelin formation [IMP]
- chromosome segregation [ISO]
- embryonic cleavage [IMP]
- erythrocyte maturation [IMP]
- extracellular matrix organization [IMP]
- hair cell differentiation [IMP, ISO]
- hair cycle process [IMP]
- hair follicle maturation [IMP]
- hematopoietic stem cell differentiation [IMP]
- in utero embryonic development [IMP]
- multicellular organism growth [IMP]
- nucleotide-excision repair [IMP, ISO]
- nucleotide-excision repair, DNA incision [ISO]
- positive regulation of DNA binding [IMP]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- post-embryonic development [IMP]
- protein phosphorylation [ISO]
- regulation of mitotic cell cycle phase transition [ISO]
- response to UV [IMP]
- response to oxidative stress [IMP, ISO]
- skin development [IMP]
- spinal cord development [IMP]
- transcription from RNA polymerase II promoter [ISO]
- transcription-coupled nucleotide-excision repair [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
An Interaction Landscape of Ubiquitin Signaling.
Intracellular signaling via the covalent attachment of different ubiquitin linkages to protein substrates is fundamental to many cellular processes. Although linkage-selective ubiquitin interactors have been studied on a case-by-case basis, proteome-wide analyses have not been conducted yet. Here, we present ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS), a quantitative interaction proteomics method that makes use of chemically synthesized diubiquitin to enrich ... [more]
Throughput
- High Throughput
Additional Notes
- #LPPI
- Chemically synthesized diubiquitin
- Likely protein-protein interaction
- Mouse embryonic stem cells (ESCs)
- Ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS)
Curated By
- BioGRID