BAIT
UBC
2700054O04Rik, AI194771, Rps27a, TI-225, Uba52, Ubb
ubiquitin C
GO Process (1)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
MAD2L1
AA673185, MAD2
MAD2 mitotic arrest deficient-like 1
GO Process (9)
GO Function (3)
GO Component (9)
Gene Ontology Biological Process
- mitotic cell cycle checkpoint [ISO]
- mitotic sister chromatid segregation [IMP]
- mitotic spindle assembly checkpoint [IMP, ISA]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of mitotic anaphase-promoting complex activity [ISO]
- negative regulation of mitotic cell cycle [ISO]
- negative regulation of mitotic metaphase/anaphase transition [TAS]
- negative regulation of protein catabolic process [ISO]
- positive regulation of mitotic cell cycle spindle assembly checkpoint [IMP, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
An Interaction Landscape of Ubiquitin Signaling.
Intracellular signaling via the covalent attachment of different ubiquitin linkages to protein substrates is fundamental to many cellular processes. Although linkage-selective ubiquitin interactors have been studied on a case-by-case basis, proteome-wide analyses have not been conducted yet. Here, we present ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS), a quantitative interaction proteomics method that makes use of chemically synthesized diubiquitin to enrich ... [more]
Mol. Cell Mar. 02, 2017; 65(5);941-955.e8 [Pubmed: 28190767]
Throughput
- High Throughput
Additional Notes
- #LPPI
- Chemically synthesized diubiquitin
- Likely protein-protein interaction
- Mouse embryonic stem cells (ESCs)
- Ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS)
Curated By
- BioGRID