BAIT

UBC

2700054O04Rik, AI194771, Rps27a, TI-225, Uba52, Ubb
ubiquitin C
GO Process (1)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

OTUB1

AI850305
OTU domain, ubiquitin aldehyde binding 1
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Mus musculus

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

An Interaction Landscape of Ubiquitin Signaling.

Zhang X, Smits AH, van Tilburg GB, Jansen PW, Makowski MM, Ovaa H, Vermeulen M

Intracellular signaling via the covalent attachment of different ubiquitin linkages to protein substrates is fundamental to many cellular processes. Although linkage-selective ubiquitin interactors have been studied on a case-by-case basis, proteome-wide analyses have not been conducted yet. Here, we present ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS), a quantitative interaction proteomics method that makes use of chemically synthesized diubiquitin to enrich ... [more]

Mol. Cell Mar. 02, 2017; 65(5);941-955.e8 [Pubmed: 28190767]

Throughput

  • High Throughput

Additional Notes

  • #LPPI
  • Chemically synthesized diubiquitin
  • Likely protein-protein interaction
  • Mouse embryonic stem cells (ESCs)
  • Ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UBC OTUB1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
1512975

Curated By

  • BioGRID