UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PDPK1
Gene Ontology Biological Process
- activation of protein kinase B activity [ISO]
- calcium-mediated signaling [ISO]
- cell migration [ISO]
- cellular response to epidermal growth factor stimulus [ISO]
- cellular response to insulin stimulus [ISO]
- epidermal growth factor receptor signaling pathway [ISO]
- extrinsic apoptotic signaling pathway [ISO]
- focal adhesion assembly [ISO]
- hyperosmotic response [IDA]
- negative regulation of cardiac muscle cell apoptotic process [IMP]
- negative regulation of protein kinase activity [ISO]
- negative regulation of toll-like receptor signaling pathway [IMP]
- negative regulation of transforming growth factor beta receptor signaling pathway [ISO]
- peptidyl-threonine phosphorylation [ISO]
- positive regulation of establishment of protein localization to plasma membrane [ISO]
- positive regulation of phospholipase activity [ISO]
- positive regulation of release of sequestered calcium ion into cytosol [ISO]
- protein phosphorylation [IDA, ISO]
- regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- regulation of endothelial cell migration [IMP]
- regulation of mast cell degranulation [IMP]
- signal transduction [TAS]
- type B pancreatic cell development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
An Interaction Landscape of Ubiquitin Signaling.
Intracellular signaling via the covalent attachment of different ubiquitin linkages to protein substrates is fundamental to many cellular processes. Although linkage-selective ubiquitin interactors have been studied on a case-by-case basis, proteome-wide analyses have not been conducted yet. Here, we present ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS), a quantitative interaction proteomics method that makes use of chemically synthesized diubiquitin to enrich ... [more]
Throughput
- High Throughput
Additional Notes
- #LPPI
- Chemically synthesized diubiquitin
- Likely protein-protein interaction
- Mouse embryonic stem cells (ESCs)
- Ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS)
Curated By
- BioGRID