Publication

An Interaction Landscape of Ubiquitin Signaling.

Zhang X, Smits AH, van Tilburg GB, Jansen PW, Makowski MM, Ovaa H, Vermeulen M

Intracellular signaling via the covalent attachment of different ubiquitin linkages to protein substrates is fundamental to many cellular processes. Although linkage-selective ubiquitin interactors have been studied on a case-by-case basis, proteome-wide analyses have not been conducted yet. Here, we present ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS), a quantitative interaction proteomics method that makes use of chemically synthesized diubiquitin to enrich ... [more]

Throughput

• High Throughput

Additional Notes

• #LPPI
• Chemically synthesized diubiquitin
• Likely protein-protein interaction
• Mouse embryonic stem cells (ESCs)
• Ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS)

Curated By

• BioGRID