Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

An Interaction Landscape of Ubiquitin Signaling.

Zhang X, Smits AH, van Tilburg GB, Jansen PW, Makowski MM, Ovaa H, Vermeulen M

Intracellular signaling via the covalent attachment of different ubiquitin linkages to protein substrates is fundamental to many cellular processes. Although linkage-selective ubiquitin interactors have been studied on a case-by-case basis, proteome-wide analyses have not been conducted yet. Here, we present ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS), a quantitative interaction proteomics method that makes use of chemically synthesized diubiquitin to enrich ... [more]

Mol. Cell Mar. 02, 2017; 65(5);941-955.e8 [Pubmed: 28190767]

Throughput

  • High Throughput

Additional Notes

  • #LPPI
  • Chemically synthesized diubiquitin
  • Likely protein-protein interaction
  • Mouse embryonic stem cells (ESCs)
  • Ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS)

Curated By

  • BioGRID