UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ITPR3
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISO]
- calcium ion transmembrane transport [ISO]
- calcium ion transport [IDA]
- calcium ion transport into cytosol [IDA]
- cellular response to cAMP [ISO]
- inositol phosphate-mediated signaling [IDA, ISO]
- long-term synaptic potentiation [IMP]
- memory [IMP]
- positive regulation of cytosolic calcium ion concentration [ISO]
- protein heterooligomerization [ISO]
- protein homooligomerization [ISO]
- response to calcium ion [ISO]
- sensory perception of bitter taste [IMP]
- sensory perception of sweet taste [IMP]
- sensory perception of umami taste [IMP]
Gene Ontology Molecular Function- calcium ion binding [ISO]
- inositol 1,3,4,5 tetrakisphosphate binding [ISO]
- inositol 1,4,5 trisphosphate binding [ISO]
- inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [IDA, ISO]
- inositol hexakisphosphate binding [ISO]
- intracellular ligand-gated calcium channel activity [IDA]
- phosphatidylinositol binding [IDA]
- protein binding [IPI]
- calcium ion binding [ISO]
- inositol 1,3,4,5 tetrakisphosphate binding [ISO]
- inositol 1,4,5 trisphosphate binding [ISO]
- inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [IDA, ISO]
- inositol hexakisphosphate binding [ISO]
- intracellular ligand-gated calcium channel activity [IDA]
- phosphatidylinositol binding [IDA]
- protein binding [IPI]
Gene Ontology Cellular Component
- apical part of cell [ISO]
- brush border [ISO]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- dendrite [ISO]
- endoplasmic reticulum [ISO]
- endoplasmic reticulum membrane [IDA, ISO]
- integral component of plasma membrane [ISO]
- membrane [ISO]
- myelin sheath [ISO]
- neuronal cell body [ISO]
- nuclear envelope [ISO]
- nuclear outer membrane [ISO]
- nucleolus [IDA]
- nucleoplasm [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
- receptor complex [ISO]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
An Interaction Landscape of Ubiquitin Signaling.
Intracellular signaling via the covalent attachment of different ubiquitin linkages to protein substrates is fundamental to many cellular processes. Although linkage-selective ubiquitin interactors have been studied on a case-by-case basis, proteome-wide analyses have not been conducted yet. Here, we present ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS), a quantitative interaction proteomics method that makes use of chemically synthesized diubiquitin to enrich ... [more]
Throughput
- High Throughput
Additional Notes
- #LPPI
- Chemically synthesized diubiquitin
- Likely protein-protein interaction
- Mouse embryonic stem cells (ESCs)
- Ubiquitin interactor affinity enrichment-mass spectrometry (UbIA-MS)
Curated By
- BioGRID