BAIT

RYR2

ARVC2, ARVD2, RYR-2, RyR, VTSIP, RP4-626J7.1
ryanodine receptor 2 (cardiac)
GO Process (35)
GO Function (13)
GO Component (9)

Gene Ontology Biological Process

Homo sapiens
PREY

PSEN1

AD3, FAD, PS-1, PS1, S182
presenilin 1
GO Process (15)
GO Function (6)
GO Component (27)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Presenilins regulate the cellular activity of ryanodine receptors differentially through isotype-specific N-terminal cysteines.

Payne AJ, Gerdes BC, Naumchuk Y, McCalley AE, Kaja S, Koulen P

Presenilins (PS), endoplasmic reticulum (ER) transmembrane proteins, form the catalytic core of γ-secretase, an amyloid precursor protein processing enzyme. Mutations in PS lead to Alzheimer's disease (AD) by altering γ-secretase activity to generate pathologic amyloid beta and amyloid plaques in the brain. Here, we identified a novel mechanism where binding of a soluble, cytosolic N-terminal domain fragment (NTF) of PS ... [more]

Exp. Neurol. Dec. 01, 2013; 250();143-50 [Pubmed: 24029002]

Throughput

  • Low Throughput

Ontology Terms

  • sh-sy5y cell (BTO:0000793)

Curated By

  • BioGRID