An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Channel-interacting PDZ protein, 'CIPP', interacts with proteins involved in cytoskeletal dynamics.

Alpi E, Landi E, Barilari M, Serresi M, Salvadori P, Bachi A, Dente L

Neuronal CIPP (channel-interacting PDZ protein) is a multivalent PDZ protein that interacts with specific channels and receptors highly expressed in the brain. It is composed of four PDZ domains that behave as a scaffold to clusterize functionally connected proteins. In the present study, we selected a set of potential CIPP interactors that are involved directly or indirectly in mechanisms of ... [more]

Biochem. J. Apr. 15, 2009; 419(2);289-300 [Pubmed: 19138174]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
INADL CNP	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Alpi E (2009)	Low	-	BioGRID	-
INADL CNP	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Alpi E (2009)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

INADL

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

CNP

Gene Ontology Biological Process