BAIT

AATK

AATYK, AATYK1, LMR1, LMTK1, PPP1R77, p35BP
apoptosis-associated tyrosine kinase
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens
PREY

PPM1K

BDP, MSUDMV, PP2Ckappa, PP2Cm, PTMP, UG0882E07
protein phosphatase, Mg2+/Mn2+ dependent, 1K
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A Global Analysis of the Receptor Tyrosine Kinase-Protein Phosphatase Interactome.

Yao Z, Darowski K, St-Denis N, Wong V, Offensperger F, Villedieu A, Amin S, Malty R, Aoki H, Guo H, Xu Y, Iorio C, Kotlyar M, Emili A, Jurisica I, Neel BG, Babu M, Gingras AC, Stagljar I

Receptor tyrosine kinases (RTKs) and protein phosphatases comprise protein families that play crucial roles in cell signaling. We used two protein-protein interaction (PPI) approaches, the membrane yeast two-hybrid (MYTH) and the mammalian membrane two-hybrid (MaMTH), to map the PPIs between human RTKs and phosphatases. The resulting RTK-phosphatase interactome reveals a considerable number of previously unidentified interactions and suggests specific roles ... [more]

Mol. Cell Jan. 19, 2017; 65(2);347-360 [Pubmed: 28065597]

Throughput

  • Low Throughput

Additional Notes

  • MYTH assay

Curated By

  • BioGRID