AATF
Gene Ontology Biological Process
- cell adhesion [ISO]
- cellular response to DNA damage stimulus [ISO]
- embryonic cleavage [ISO]
- negative regulation of amyloid precursor protein biosynthetic process [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of reactive oxygen species metabolic process [ISO]
- negative regulation of superoxide anion generation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of mitotic cell cycle [ISO]
- ribosome biogenesis [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- centrosome [ISO]
- cytoplasm [IDA, ISO]
- focal adhesion [ISO]
- nucleolus [ISO]
- nucleus [IDA, ISO]
BLOC1S2
Gene Ontology Biological Process
- anterograde axon cargo transport [ISO, ISS]
- anterograde synaptic vesicle transport [ISO, ISS]
- extrinsic apoptotic signaling pathway via death domain receptors [ISO, ISS]
- mitochondrial outer membrane permeabilization [ISO, ISS]
- neuron projection development [ISS]
- positive regulation of cell proliferation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription, DNA-templated [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Characterization of rat BLOS2/Ceap, a putative yeast She3 homolog, as interaction partner of apoptosis antagonizing transcription factor/Che-1.
AATF/Che-1 is a coactivator of several transcription factors, including steroid hormone receptors. In search of novel interaction partners of AATF, we identified BLOS2 (BLOC1S2, also termed Ceap) from a rat cDNA library. BLOS2 is extremely conserved with a high degree of homology to yeast She3p. The clone isolated represents a splice variant encoding a polypeptide of 168 residues. Rat BLOS2 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AATF BLOC1S2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| AATF BLOC1S2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID