PREY

TAF1

BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, N-TAF1, NSCL2, OF, P250, TAF(II)250, TAF2A, TAFII-250, TAFII250, XDP
TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Brain proteins interacting with the tetramerization region of non-erythroid alpha spectrin.

Oh Y, Fung LW

The N-terminal region of non-erythroid alpha spectrin (Sp alpha II) is responsible for interacting with its binding partner, beta spectrin, to form functional spectrin tetramers. We used a yeast-two-hybrid system, with an N-terminal segment of alpha spectrin representing the functional tetramerization site, as a bait to screen human brain c-DNA library for proteins that interact with the alpha spectrin segment. ... [more]

Cell. Mol. Biol. Lett. Jul. 04, 2007; 12(4);604-20 [Pubmed: 17607528]

Throughput

  • Low Throughput

Curated By

  • BioGRID