Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Mextli is a novel eukaryotic translation initiation factor 4E-binding protein that promotes translation in Drosophila melanogaster.

Hernandez G, Miron M, Han H, Liu N, Magescas J, Tettweiler G, Frank F, Siddiqui N, Sonenberg N, Lasko P

Translation is a fundamental step in gene expression, and translational control is exerted in many developmental processes. Most eukaryotic mRNAs are translated by a cap-dependent mechanism, which requires recognition of the 5'-cap structure of the mRNA by eukaryotic translation initiation factor 4E (eIF4E). eIF4E activity is controlled by eIF4E-binding proteins (4E-BPs), which by competing with eIF4G for eIF4E binding act ... [more]

Mol. Cell. Biol. Aug. 01, 2013; 33(15);2854-64 [Pubmed: 23716590]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MXT EIF4E-7	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Giot L (2003)	High	-	BioGRID	-

Curated By

FlyBase

Interaction Summary

EIF4E-7

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA 7-methylguanosine cap binding [IDA]RNA cap binding [ISS, NAS]eukaryotic initiation factor 4G binding [IPI]protein binding [IPI]translation initiation factor activity [IGI, ISS, NAS]

Gene Ontology Cellular Component

MXT