BAIT

NDC80

CG9938, Dmel\CG9938, Hec1, dNdc80, dmNdc80, Dmel_CG9938
CG9938 gene product from transcript CG9938-RA
GO Process (5)
GO Function (0)
GO Component (2)
Drosophila melanogaster
PREY

NUF2

CG8902, Dmel\CG8902, anon-WO0118547.214, dmNuf2, l(2)SH2 2276, Dmel_CG8902
CG8902 gene product from transcript CG8902-RA
GO Process (4)
GO Function (0)
GO Component (2)
Drosophila melanogaster

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Molecular analysis of core kinetochore composition and assembly in Drosophila melanogaster.

Przewloka MR, Zhang W, Costa P, Archambault V, D'Avino PP, Lilley KS, Laue ED, McAinsh AD, Glover DM

BACKGROUND: Kinetochores are large multiprotein complexes indispensable for proper chromosome segregation. Although Drosophila is a classical model organism for studies of chromosome segregation, little is known about the organization of its kinetochores. METHODOLOGY/PRINCIPAL FINDINGS: We employed bioinformatics, proteomics and cell biology methods to identify and analyze the interaction network of Drosophila kinetochore proteins. We have shown that three Drosophila proteins ... [more]

PLoS ONE May. 31, 2007; 2(5);e478 [Pubmed: 17534428]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NUF2 NDC80
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-FlyBase
-
NUF2 NDC80
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By