PAR-6
Gene Ontology Biological Process
- apical protein localization [NAS]
- asymmetric neuroblast division [IGI]
- border follicle cell migration [IMP]
- branching involved in open tracheal system development [IMP]
- cell adhesion [IMP]
- epithelium development [IMP]
- establishment of neuroblast polarity [IMP]
- establishment or maintenance of epithelial cell apical/basal polarity [NAS, TAS]
- establishment or maintenance of neuroblast polarity [NAS]
- establishment or maintenance of polarity of embryonic epithelium [NAS]
- germarium-derived female germ-line cyst formation [IMP]
- morphogenesis of a polarized epithelium [TAS]
- negative regulation of protein kinase activity [IDA]
- oocyte axis specification [TAS]
- phagocytosis [IMP]
- positive regulation of filopodium assembly [IMP]
- positive regulation of lamellipodium assembly [IMP]
- regulation of cell shape [IMP]
- synapse assembly [IMP]
- zonula adherens assembly [NAS, TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
APKC
Gene Ontology Biological Process
- apical protein localization [NAS]
- asymmetric neuroblast division [IGI]
- asymmetric protein localization involved in cell fate determination [IPI]
- branching involved in open tracheal system development [IMP]
- cell-cell junction assembly [NAS]
- compound eye retinal cell programmed cell death [IDA]
- epithelial cell morphogenesis [IGI, IMP]
- establishment of epithelial cell apical/basal polarity [IGI]
- establishment of epithelial cell planar polarity [IGI, IMP]
- establishment of mitotic spindle orientation [IMP]
- establishment or maintenance of cell polarity [IMP, NAS]
- establishment or maintenance of epithelial cell apical/basal polarity [IMP, NAS, TAS]
- establishment or maintenance of neuroblast polarity [IGI, IMP, NAS]
- establishment or maintenance of polarity of embryonic epithelium [IMP, NAS]
- establishment or maintenance of polarity of follicular epithelium [IMP]
- germarium-derived oocyte fate determination [IMP]
- maintenance of cell polarity [IMP]
- melanotic encapsulation of foreign target [IMP]
- memory [IMP]
- morphogenesis of a polarized epithelium [TAS]
- oocyte anterior/posterior axis specification [IGI]
- oocyte axis specification [TAS]
- positive regulation of filopodium assembly [IMP]
- positive regulation of lamellipodium assembly [IMP]
- positive regulation of neuroblast proliferation [IMP]
- protein phosphorylation [NAS]
- sensory organ development [IMP]
- spermatogenesis [IMP]
- synapse assembly [IMP]
- zonula adherens assembly [NAS, TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Stringent analysis of gene function and protein-protein interactions using fluorescently tagged genes.
In Drosophila collections of green fluorescent protein (GFP) trap lines have been used to probe the endogenous expression patterns of trapped genes or the subcellular localization of their protein products. Here, we describe a method, based on nonoverlapping, highly specific, shRNA transgenes directed against GFP, that extends the utility of these collections to loss-of-function studies. Furthermore, we used a MiMIC ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
PAR-6 APKC | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | FlyBase | - | |
APKC PAR-6 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | FlyBase | - | |
PAR-6 APKC | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | FlyBase | 2313531 | |
PAR-6 APKC | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | FlyBase | - | |
PAR-6 APKC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
PAR-6 APKC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
PAR-6 APKC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
PAR-6 APKC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
APKC PAR-6 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
PAR-6 APKC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
PAR-6 APKC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
APKC PAR-6 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
APKC PAR-6 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |