SLP1
Gene Ontology Biological Process
- anterior/posterior axis specification, embryo [NAS]
- axon midline choice point recognition [IBA]
- blastoderm segmentation [TAS]
- dorsal/ventral pattern formation [IBA]
- embryo development ending in birth or egg hatching [IBA]
- heart development [TAS]
- heart formation [IMP]
- mesoderm formation [TAS]
- negative regulation of neuron differentiation [IBA]
- negative regulation of transcription from RNA polymerase II promoter [IBA, IDA]
- neuron fate specification [IBA]
- nonmotile primary cilium assembly [IBA]
- positive regulation of cell cycle [IBA]
- positive regulation of neuroblast proliferation [IBA]
- positive regulation of transcription from RNA polymerase II promoter [IBA]
- regulation of gene expression [IMP]
- regulation of mitotic cell cycle [IBA]
- regulation of sequence-specific DNA binding transcription factor activity [IBA]
- regulation of transcription, DNA-templated [ISS]
- segment specification [NAS]
- specification of segmental identity, head [IEP, IGI, IMP]
- specification of segmental identity, mandibular segment [NAS]
- tissue development [IBA]
Gene Ontology Molecular Function- DNA binding, bending [IBA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IBA, IDA]
- double-stranded DNA binding [IBA]
- enhancer sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription factor binding [IBA]
- DNA binding, bending [IBA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IBA, IDA]
- double-stranded DNA binding [IBA]
- enhancer sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription factor binding [IBA]
GRO
Gene Ontology Biological Process
- actin filament organization [IMP]
- dendrite morphogenesis [IMP]
- lateral inhibition [IMP]
- negative regulation of Wnt signaling pathway [NAS]
- negative regulation of canonical Wnt signaling pathway [IDA]
- negative regulation of smoothened signaling pathway [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IPI, TAS]
- negative regulation of transcription, DNA-templated [IDA, IGI, NAS, TAS]
- nervous system development [IMP, NAS]
- regulation of transcription, DNA-templated [IGI, IMP]
- sex determination, primary response to X:A ratio [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- nucleus [NAS, TAS]
- protein complex [IPI]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Groucho augments the repression of multiple Even skipped target genes in establishing parasegment boundaries.
Groucho acts as a co-repressor for several Drosophila DNA binding transcriptional repressors. Several of these proteins have been found to contain both Groucho-dependent and -independent repression domains, but the extent to which this distinction has functional consequences for the regulation of different target genes is not known. The product of the pair-rule gene even skipped has previously been shown to ... [more]
Throughput
- Low Throughput