BAIT

MIO

154826_at, CG18362, Dmel\CG18362, Mondo, anon-39Da, bHLHd12, dmondo, Dmel_CG18362
Mlx interactor
Drosophila melanogaster
PREY

BIGMAX

CG3350, DmMlx, Dmel\CG3350, Mlx, bHLHd13, Dmel_CG3350
CG3350 gene product from transcript CG3350-RA
Drosophila melanogaster

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Mondo/ChREBP-Mlx-regulated transcriptional network is essential for dietary sugar tolerance in Drosophila.

Havula E, Teesalu M, Hyoetylaeinen T, Seppaelae H, Hasygar K, Auvinen P, Oresic M, Sandmann T, Hietakangas V

Sugars are important nutrients for many animals, but are also proposed to contribute to overnutrition-derived metabolic diseases in humans. Understanding the genetic factors governing dietary sugar tolerance therefore has profound biological and medical significance. Paralogous Mondo transcription factors ChREBP and MondoA, with their common binding partner Mlx, are key sensors of intracellular glucose flux in mammals. Here we report analysis ... [more]

PLoS Genet. Apr. 01, 2013; 9(4);e1003438 [Pubmed: 23593032]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MIO BIGMAX
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By