BAIT

DREP-1

CG8357, DREP-1/dICAD, Dmel\CG8357, Drep1, ICAD, ICAD/DREP-1, Rep1, dICAD, Dmel_CG8357
DNA fragmentation factor-related protein 1
Drosophila melanogaster
PREY

DREP-2

CG1975, Dmel\CG1975, Drep2, Rep2, anon-WO0118547.162, anon-WO0118547.168, Dmel_CG1975
DNA fragmentation factor-related protein 2
GO Process (2)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Drosophila melanogaster

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Genes with homology to DFF/CIDEs found in Drosophila melanogaster.

Inohara N, Nunez G

... [more]

Cell Death Differ. Sep. 01, 1999; 6(9);823-4 [Pubmed: 10627165]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DREP-1 DREP-2
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Low-FlyBase
-
DREP-1 DREP-2
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Low-FlyBase
-
DREP-1 DREP-2
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Low-FlyBase
-
DREP-1 DREP-2
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Low-FlyBase
-
DREP-2 DREP-1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By